Association of proinflammatory cytokine tumor necrosis factor-α gene promoter -308 and -238 polymorphism with psoriasis in North Indian population
Wani Aadil1, Bashir Ahmad Ganai2, Tahseena Akhtar3, Tarun Narang4, Rajinder Kaur3
1 Department of Human Genetics, Punjabi University, Patiala, Punjab; Department of Biochemistry, University of Kashmir, Srinagar, Jammu and Kashmir, India
2 Center of Research for Development (CORD), University of Kashmir, Srinagar, Jammu and Kashmir, India
3 Department of Human Genetics, Punjabi University, Patiala, Punjab, India
4 Department of Dermatology, Venereology and Leprology, Post Graduate Institute of Medical Education and Research, Chandigarh, India
Department of Human Genetics, Punjabi University, Patiala, Punjab
Source of Support: None, Conflict of Interest: None
Background/objective Tumor necrosis factor (TNF) is an important proinflammatory cytokine that plays a role in the pathogenesis of psoriasis. The aim of the present study was to investigate the role of TNF-α −308G/A and TNF-α −238G/A polymorphism, haplotype, and serum level in the pathogenesis of psoriasis.
Materials and methods A total of 200 psoriatic patients and 200 controls were genotyped for TNF-α −308G/A and TNF–α −238G/A polymorphism by using polymerase chain reaction. In addition, serum levels of TNF-α were measured by enzyme-linked immunosorbent assay.
Results Polymorphism of TNF–α −308 was found to be associated with a decreased risk for psoriasis odds ratio=0.29; (95% confidence interval=0.14–0.62), and polymorphism of TNF–α −238 was associated with an increased risk for psoriasis odds ratio=37.81; (95% confidence interval=12.77–112.01). HT2 GA haplotype was found to be associated with an increased risk for psoriasis. Moreover, the serum TNF-α level increased in patients compared with controls, with a significant correlation between serum TNF-α and psoriasis severity.
Conclusion Our findings suggested that TNF-α polymorphisms imparted significant risk toward the development of psoriasis in north Indian population.